Assessment of Immunotoxicity and Oxidative Stress Induced by Zinc Selenium/Zinc Sulphide Quantum Dots

Although ZnSe/ZnS quantum dots (QDs) have emerged as apparently less hazardous substitute to cadmium-based QDs, their toxicity has not been fully understood. Huge levels of ROS production and associated difficulties comprise the underlying reason for nanomaterial in cells. This will cause both immunotoxicity genotoxicity. In current work, Zinc Selenium/Zinc Sulphide (ZnSe/ZnS) QDs was synthesized, characterized analyzed its role oxidative stress induction two cell lines (HepG2 HEK) Swiss Albino mice. influence catalase activity measured by DCFHDA assay HepG2 HEK cells after exposure QDs. Assessment nitrile radical formation carried out griess reagent. Level GSH is assessed a marker induced Cell death investigated Calcein AM-PI live dead assay. Apoptotic DNA ladder studying potential induce fragmentation. vivo bio-nano interaction studied exposing mice via i.v. i.p. injection. Antioxidant assays were brain liver homogenates study stress. LPO, GSH, GPx, GR SOD are considered biomarkers analysis. Blood barrier (BBB) integrity also studied. Spleenocytes proliferation response. do visible upto concentration 50 μg/ml. occurs at higher (100 μg/ml) caused production. Overall provide attentive information that capable eliciting any serious damages tissues which turn substantiates applicability biomedical applications.